| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
|
|
|
|
|||||||||||||
|
|
|||||||||||||||
B of cytokine induced expression of
intercellular adhesion molecule 1 (ICAM-1) in an intestinal epithelial
cell line, a process blocked by proteasome inhibitors
C Jobin Departments of Medicine and the Center for
Gastrointestinal Biology and Disease, University of North Carolina at
Chapel Hill, Chapel Hill, North Carolina 27599, USA
Correspondence to: Dr R B Sartor,
Division of Digestive Diseases and Nutrition, CB# 7080, Burnett-Womack
Bldg, University of North Carolina at Chapel Hill, Chapel Hill, NC
27599-7080, USA. Accepted for publication 18 December 1997 Background/aims Keywords:
adhesion molecules;
ICAM-1;
cytokines;
tumour
necrosis factor
The gene promoter for the
intercellular adhesion molecule ICAM-1 possesses binding sites for
several transcriptional factors, including nuclear factor 
B
(NF-B). The role of NF-B in ICAM-1 gene regulation was therefore
examined by using different proteasome inhibitors in tumour necrosis
factor 
(TNF-) stimulated IEC-6 rat intestinal epithelial cells.
MethodsICAM-1 expression was analysed by enzyme
linked immunosorbent assay (ELISA), reverse transcriptase polymerase
chain reaction, and immunohistochemistry. Steady state levels of
cytoplasmic IB protein were evaluated by western blot, and nuclear
translocation of NF-B was determined by electrophoretic mobility
shift assay and immunofluorescence staining. Cell adhesion was assayed
by measuring the binding of fluorescence labelled MOLT-4 cells.
ResultsTNF- induced ICAM-1 mRNA and protein
expression in IEC-6 cells, which was followed by increased adhesion of
MOLT-4 lymphocytes. Blocking TNF- induced IB degradation with
proteasome inhibitors reduced TNF- induced NF-B activation and
ICAM-1 gene induction and notably decreased MOLT-4 cell adhesion
without affecting Jun N-terminal kinase (JNK/SAPK) activity or de novo
protein synthesis.
ConclusionTNF- induction of ICAM-1 expression
is mediated by the transcription factor NF-B and can be inhibited by
blocking IB degradation. Thus the IB/NF-B system is a
promising target for pharmacological modulation of the expression of
adhesion molecules and other inflammatory genes in the intestine.
(GUT 1998;42:779-787)
;
intestinal inflammation;
NF-B.
© 1998 by Gut
This article has been cited by other articles:
|
|
 |
|
  R. Bhowmick, A. Ghosal, B. Das, H. Koley, D. R. Saha, S. Ganguly, R. K. Nandy, R. K. Bhadra, and N. S. Chatterjee Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin Infect. Immun., November 1, 2008; 76(11): 4968 - 4977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C Moss, P. Anton, T. Savidge, P. Newman, A. S Cheifetz, J. Gay, S. Paraschos, M. W. Winter, M. P Moyer, K. Karalis, et al. Urocortin II mediates pro-inflammatory effects in human colonocytes via corticotropin-releasing hormone receptor 2{alpha} Gut, September 1, 2007; 56(9): 1210 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chanchevalap, M. O. Nandan, B. B. McConnell, L. Charrier, D. Merlin, J. P. Katz, and V. W. Yang Kruppel-like factor 5 is an important mediator for lipopolysaccharide-induced proinflammatory response in intestinal epithelial cells Nucleic Acids Res., February 25, 2006; 34(4): 1216 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, B. Walia, J. Evans, A. T. Gewirtz, D. Merlin, and S. V. Sitaraman IL-6 Induces NF-{kappa}B Activation in the Intestinal Epithelia J. Immunol., September 15, 2003; 171(6): 3194 - 3201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Coeffier, S. Claeyssens, B. Hecketsweiler, A. Lavoinne, P. Ducrotte, and P. Dechelotte Enteral glutamine stimulates protein synthesis and decreases ubiquitin mRNA level in human gut mucosa Am J Physiol Gastrointest Liver Physiol, July 7, 2003; 285(2): G266 - G273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Courtois, E. G Seidman, E. Delvin, C. Asselin, S. Bernotti, M. Ledoux, and E. Levy Membrane peroxidation by lipopolysaccharide and iron-ascorbate adversely affects Caco-2 cell function: beneficial role of butyric acid Am. J. Clinical Nutrition, March 1, 2003; 77(3): 744 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Haller, M. P. Russo, R. B. Sartor, and C. Jobin IKKbeta and Phosphatidylinositol 3-Kinase/Akt Participate in Non-pathogenic Gram-negative Enteric Bacteria-induced RelA Phosphorylation and NF-kappa B Activation in Both Primary and Intestinal Epithelial Cell Lines J. Biol. Chem., October 4, 2002; 277(41): 38168 - 38178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhong, M. Froh, H. D. Connor, X. Li, L. O. Conzelmann, R. P. Mason, J. J. Lemasters, and R. G. Thurman Prevention of hepatic ischemia-reperfusion injury by green tea extract Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G957 - G964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. B. Jijon, W. J. Panenka, K. L. Madsen, and H. G. Parsons MAP kinases contribute to IL-8 secretion by intestinal epithelial cells via a posttranscriptional mechanism Am J Physiol Cell Physiol, July 1, 2002; 283(1): C31 - C41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S J H van Deventer Small therapeutic molecules for the treatment of inflammatory bowel disease Gut, May 1, 2002; 50(90003): iii47 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Pritts, E. S. Hungness, D. D. Hershko, B. W. Robb, X. Sun, G.-J. Luo, J. E. Fischer, H. R. Wong, and P.-O. Hasselgren Proteasome inhibitors induce heat shock response and increase IL-6 expression in human intestinal epithelial cells Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1016 - R1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, B. U. Bradford, M. D. Wheeler, S. A. Stimpson, H. M. Pink, T. A. Brodie, J. H. Schwab, and R. G. Thurman Dietary Glycine Prevents Peptidoglycan Polysaccharide-Induced Reactive Arthritis in the Rat: Role for Glycine-Gated Chloride Channel Infect. Immun., September 1, 2001; 69(9): 5883 - 5891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cecilia Berin, L. Eckmann, D. H. Broide, and M. F. Kagnoff Regulated Production of the T Helper 2-Type T-Cell Chemoattractant TARC by Human Bronchial Epithelial Cells In Vitro and in Human Lung Xenografts Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 382 - 389. [Abstract] [Full Text]
|
|
|
|
|
|
H. Lum and K. A. Roebuck Oxidant stress and endothelial cell dysfunction Am J Physiol Cell Physiol, April 1, 2001; 280(4): C719 - C741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Blinman, I. Gukovsky, M. Mouria, V. Zaninovic, E. Livingston, S. J. Pandol, and A. S. Gukovskaya Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1993 - C2003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Zaninovic, A. S. Gukovskaya, I. Gukovsky, M. Mouria, and S. J. Pandol Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells Am J Physiol Gastrointest Liver Physiol, October 1, 2000; 279(4): G666 - G676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hatano, C. A. Bradham, A. Stark, Y. Iimuro, J. J. Lemasters, and D. A. Brenner The Mitochondrial Permeability Transition Augments Fas-induced Apoptosis in Mouse Hepatocytes J. Biol. Chem., April 14, 2000; 275(16): 11814 - 11823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Bocker, A. Schottelius, J. M. Watson, L. Holt, L. L. Licato, D. A. Brenner, R. B. Sartor, and C. Jobin Cellular Differentiation Causes a Selective Down-regulation of Interleukin (IL)-1beta -mediated NF-kappa B Activation and IL-8 Gene Expression in Intestinal Epithelial Cells J. Biol. Chem., April 14, 2000; 275(16): 12207 - 12213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jobin and R. B. Sartor The Ikappa B/NF-kappa B system: a key determinant of mucosal inflammation and protection Am J Physiol Cell Physiol, March 1, 2000; 278(3): C451 - C462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. G. Schottelius, M. W. Mayo, R. B. Sartor, and A. S. Baldwin Jr. Interleukin-10 Signaling Blocks Inhibitor of kappa B Kinase Activity and Nuclear Factor kappa B DNA Binding J. Biol. Chem., November 5, 1999; 274(45): 31868 - 31874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jobin, C. A. Bradham, M. P. Russo, B. Juma, A. S. Narula, D. A. Brenner, and R. B. Sartor Curcumin Blocks Cytokine-Mediated NF-{kappa}B Activation and Proinflammatory Gene Expression by Inhibiting Inhibitory Factor I-{kappa}B Kinase Activity J. Immunol., September 15, 1999; 163(6): 3474 - 3483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jobin, L. Holt, C. A. Bradham, K. Streetz, D. A. Brenner, and R. B. Sartor TNF Receptor-Associated Factor-2 Is Involved in Both IL-1{beta} and TNF-{alpha} Signaling Cascades Leading to NF-{kappa}B Activation and IL-8 Expression in Human Intestinal Epithelial Cells J. Immunol., April 15, 1999; 162(8): 4447 - 4454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M F NEURATH, C BECKER, and K BARBULESCU Role of NF-kappa B in immune and inflammatory responses in the gut Gut, December 1, 1998; 43(6): 856 - 860. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
