| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
|
|
|
|
|||||||||||||
|
|
|||||||||||||||
H Ueyama
a Second Department of
Internal Medicine, Osaka University Medical School, 2-2 Yamadaoka,
Suita, Osaka 565, Japan, b Department of Cancer Epidemiology, Research
Institute, Osaka Medical Center for Cancer and Cardiovascular Diseases,
3-3 Nakamichi 1-chome Higashinari-ku, Osaka 537, Japan, c First Department of
Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka
565, Japan, d Department
of Genetics, Osaka University Medical School, 2-2 Yamadaoka, Suita,
Osaka 565, Japan
Correspondence to: Dr H
Ueyama. Accepted for publication 4 February 1998 Background Keywords:
Fas ligand;
apoptosis;
ulcerative colitis;
reverse
transcription polymerase chain reaction;
T lymphocytes
The
pathogenesis of ulcerative colitis is unclear, but cytotoxic T
lymphocytes infiltrating the mucosa have been implicated in mucosal
damage. The Fas ligand (FasL), expressed on cytotoxic T lymphocytes,
induces apoptosis in cells expressing Fas.
AimTo analyse FasL
expression in affected colonic mucosa to ascertain Fas-FasL interaction
in ulcerative colitis.
MethodsFasL mRNA was
quantified in colonic mucosal specimens from healthy subjects and
patients with ulcerative colitis or Crohn's disease, using the
competitive reverse transcription polymerase chain reaction. FasL mRNA
localisation was determined by in situ hybridisation. Expression of Fas
in colonic mucosa was analysed immunohistochemically. Phenotypes of
lamina propria lymphocytes that expressed FasL were analysed by flow cytometry.
ResultsFasL mRNA was
strongly expressed in active ulcerative colitis lesions, but not in
those associated with active Crohn's disease or active proctitis-type
ulcerative colitis. In situ hybridisation showed that FasL mRNA
expression occurred in mononuclear cells infiltrating lesions. Fas was
expressed in epithelial cells in ulcerative colitis and Crohn's
disease, and in normal subjects. Cytometry showed that FasL was
expressed in CD3 lymphocytes infiltrating the lamina propria in active lesions.
ConclusionsFasL is
expressed in CD3 lymphocytes infiltrating into ulcerative colitis but
not Crohn's disease lesions, suggesting that Fas-FasL induced
apoptosis participates in the mucosal damage of ulcerative colitis.
(GUT 1998;43:48-55)
Relevant Article
This article has been cited by other articles:
|
|
 |
|
  K. M. Naugler, K. A. Baer, and M. J. Ropeleski Interleukin-11 antagonizes Fas ligand-mediated apoptosis in IEC-18 intestinal epithelial crypt cells: role of MEK and Akt-dependent signaling Am J Physiol Gastrointest Liver Physiol, March 1, 2008; 294(3): G728 - G737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Miura, M. Yamamoto, M. Fukutake, N. Ohtake, S. Iizuka, A. Ishige, H. Sasaki, K. Fukuda, T. Yamamoto, and S. Hayakawa Anti-CD3 induces bi-phasic apoptosis in murine intestinal epithelial cells: possible involvement of the Fas/Fas ligand system in different T cell compartments Int. Immunol., May 1, 2005; 17(5): 513 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Sturm, A Z A Leite, S Danese, K A Krivacic, G A West, S Mohr, J W Jacobberger, and C Fiocchi Divergent cell cycle kinetics underlie the distinct functional capacity of mucosal T cells in Crohn's disease and ulcerative colitis Gut, November 1, 2004; 53(11): 1624 - 1631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cippitelli, C. Fionda, D. Di Bona, A. Lupo, M. Piccoli, L. Frati, and A. Santoni The Cyclopentenone-Type Prostaglandin 15-Deoxy-{Delta}12,14-Prostaglandin J2 Inhibits CD95 Ligand Gene Expression in T Lymphocytes: Interference with Promoter Activation Via Peroxisome Proliferator-Activated Receptor-{gamma}-Independent Mechanisms J. Immunol., May 1, 2003; 170(9): 4578 - 4592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Pinkoski, N. M. Droin, and D. R. Green Tumor Necrosis Factor alpha Up-regulates Non-lymphoid Fas-ligand following Superantigen-induced Peripheral Lymphocyte Activation J. Biol. Chem., October 25, 2002; 277(44): 42380 - 42385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Ohman, L Franzen, U Rudolph, L Birnbaumer, and E H. Hornquist Regression of Peyer's patches in G{alpha}i2 deficient mice prior to colitis is associated with reduced expression of Bcl-2 and increased apoptosis Gut, September 1, 2002; 51(3): 392 - 397. [Abstract] [Full Text]
|
|
|
|
|
|
M Merger, J L Viney, R Borojevic, D Steele-Norwood, P Zhou, D A Clark, R Riddell, R Maric, E R Podack, and K Croitoru Defining the roles of perforin, Fas/FasL, and tumour necrosis factor {alpha} in T cell induced mucosal damage in the mouse intestine Gut, August 1, 2002; 51(2): 155 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Jones, A. S. Day, H. Jennings, P. T. Shannon, E. Galindo-Mata, and P. M. Sherman Enhanced Disease Severity in Helicobacter pylori-Infected Mice Deficient in Fas Signaling Infect. Immun., May 1, 2002; 70(5): 2591 - 2597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Martin and A. Panja Cytokine regulation of human intestinal primary epithelial cell susceptibility to Fas-mediated apoptosis Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G92 - G104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Di Sabatino, R Ciccocioppo, S D'Alo, R Parroni, D Millimaggi, M G Cifone, and G R Corazza Intraepithelial and lamina propria lymphocytes show distinct patterns of apoptosis whereas both populations are active in Fas based cytotoxicity in coeliac disease Gut, September 1, 2001; 49(3): 380 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fiorucci, E. Distrutti, M. N. Ajuebor, A. Mencarelli, R. Mannucci, B. Palazzetti, P. Del Soldato, A. Morelli, and J. L. Wallace NO-mesalamine protects colonic epithelial cells against apoptotic damage induced by proinflammatory cytokines Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G654 - G665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Connolly, Y. H. Cho, R. Duan, J. Fikes, T. Gregorio, D. W. LaFleur, Z. Okoye, T. W. Salcedo, G. Santiago, S. Ullrich, et al. In Vivo Inhibition of Fas Ligand-Mediated Killing by TR6, a Fas Ligand Decoy Receptor J. Pharmacol. Exp. Ther., July 1, 2001; 298(1): 25 - 33. [Abstract] [Full Text]
|
|
|
|
|
|
J Itoh, C de la Motte, S A Strong, A D Levine, and C Fiocchi Decreased Bax expression by mucosal T cells favours resistance to apoptosis in Crohn's disease Gut, July 1, 2001; 49(1): 35 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Le'Negrate, E. Selva, P. Auberger, B. Rossi, and P. Hofman Sustained Polymorphonuclear Leukocyte Transmigration Induces Apoptosis in T84 Intestinal Epithelial Cells J. Cell Biol., September 18, 2000; 150(6): 1479 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Iimura, T Nakamura, S Shinozaki, B Iizuka, Y Inoue, S Suzuki, and N Hayashi Bax is downregulated in inflamed colonic mucosa of ulcerative colitis Gut, August 1, 2000; 47(2): 228 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pinkoski, T. Brunner, D. R. Green, and T. Lin Fas and Fas ligand in gut and liver Am J Physiol Gastrointest Liver Physiol, March 1, 2000; 278(3): G354 - G366. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
