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G Monteleone Dipartimento di Medicina Sperimentale e Clinica,
Università di Catanzaro, Catanzaro, Italy
Correspondence to: Dr F Pallone, Cattedra di
Gastroenterologia, Dipartimento di Medicina Sperimentale, Policlinico
Universitario, Via T Campanella, 88100 Catanzaro, Italy.
Accepted for publication 13 May 1998 Background/Aim Keywords:
interleukin 12;
interleukin 7;
interleukin 15;
interferon
Interleukin (IL) 12 is involved in
the mucosal response during intestinal inflammation but its role is not fully understood. The response of human lamina propria T lymphocytes (T-LPL) to IL-12 in terms of interferon 
(IFN-) release and proliferation was investigated, exploring whether IL-15 and IL-7 cooperate with IL-12. The role of accessory molecules (CD2 and CD28)
was also investigated.
MethodsUnstimulated and phytohaemagglutinin
preactivated T-LPL cultures were incubated with or without the initial
addition of cytokines, anti-CD2 or anti-CD28 antibodies. IFN- mRNA
was detected by reverse transcriptase polymerase chain reaction, and protein secretion was measured by enzyme linked immunosorbent assay (ELISA).
ResultsIFN- mRNA was induced in T-LPLs by
IL-12 and IL-15 but not IL-7, whereas IFN- was measured only in
IL-12 stimulated T-LPL cultures. IL-12 induced IFN- release was not
abrogated by neutralising anti-IL-2 antibody or by cyclosporin A. IL-12 synergised with either anti-CD2 or anti-CD28 antibodies in inducing IFN- synthesis. In preactivated T-LPLs, IL-7 enhanced IFN-
release induced by both IL-12 and anti-CD2, whereas IL-15 potentiated only IL-12 induced IFN- synthesis. IL-12 did not induce
proliferation of either unstimulated or preactivated T-LPLs and it did
not enhance the CD2/CD28 stimulated T-LPL proliferative response. No
transcript for IL-12 receptor 
1 subunit was detected in
freshly isolated and activated T-LPLs whereas the 2
subunit mRNA was consistently found in T-LPL samples.
ConclusionsIL-12 induces human T-LPLs to produce
and release IFN-, and IL-15 and IL-7 cooperate with IL-12 in
expanding the IFN- mucosal response.
(GUT 1998;43:620-628)
;
intestinal inflammation
© 1998 by Gut
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