Gut

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER


[Advanced]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this link to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Add article to my folders
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duchmann, R
Right arrow Articles by zum Büschenfelde, K-H M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duchmann, R
Right arrow Articles by zum Büschenfelde, K-H M.
Topic Collections
Right arrowRelevant Articles
Gut 1999;44:812-818 ( June )

Article

T cell specificity and cross reactivity towards enterobacteria, Bacteroides, Bifidobacterium, and antigens from resident intestinal flora in humans R Duchmann, E May, M Heike, P Knolle, M Neurath, K-H Meyer zum Büschenfelde

I Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität Mainz, Langenbeckstrasse 1, D-55101 Mainz, Germany

Correspondence to: Professor Meyer zum   Büschenfelde.

Accepted for publication 13 January 1999

BACKGROUND---T cell responses to normal intestinal bacteria or their products may be important in the immunopathogenesis of chronic enterocolitis.
AIMS---To investigate the T cell specificity and cross reactivity towards intestinal bacteria.
PATIENTS/METHODS---T cell clones were isolated with phytohaemagglutinin from peripheral blood and biopsy specimens of inflamed and non-inflamed colon from five patients with inflammatory bowel disease (IBD) and two controls. T cell clones were restimulated with anaerobic Bacteroides and Bifidobacteria species, enterobacteria, and direct isolates of aerobic intestinal flora. T cell phenotype was analysed by single-cell immunocyte assay.
RESULTS---Analysis of 96 T cell clones isolated from peripheral blood and biopsy specimens from two patients with IBD showed that both Bifidobacterium and Bacteroides species specifically stimulate proliferation of CD4+TCRalpha beta + T cell clones from both sites and that cross reactivity exists between these anaerobic bacteria and different enterobacteria. Analysis of 210 T cell clones isolated from three patients with IBD and two controls showed that indigenous aerobic flora specifically stimulate T cell clones from peripheral blood and biopsy specimens from a foreign subject. Some of these flora specific T cell clones were cross reactive with defined enterobacteria. In addition, T cell clones stimulated by their own indigenous aerobic flora were identified in patients with IBD.
CONCLUSION---Immune responses to antigens from the intestinal microflora involve a complex network of T cell specificities.


Keywords: inflammatory bowel disease; T cells; intestinal flora; mucosal immunity

© 1999 by Gut

Relevant Articles

Anticipating the onset of inflammatory bowel disease
J F COLOMBEL, D LAHARIE, and B GRANDBASTIEN
Gut 1999 44: 773-774. [Extract] [Full Text] [PDF]

Intolerance of the dirty intestine
A J S MACPHERSON, K J MALOY, and I BJARNASON
Gut 1999 44: 774-775. [Extract] [Full Text]



This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Egawa, H. Iijima, S. Shinzaki, S. Nakajima, J. Wang, J. Kondo, S. Ishii, T. Yoshio, T. Irie, T. Nishida, et al.
Upregulation of GRAIL is associated with remission of ulcerative colitis
Am J Physiol Gastrointest Liver Physiol, July 1, 2008; 295(1): G163 - G169.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
L. Frolova, P. Drastich, P. Rossmann, K. Klimesova, and H. Tlaskalova-Hogenova
Expression of Toll-like Receptor 2 (TLR2), TLR4, and CD14 in Biopsy Samples of Patients With Inflammatory Bowel Diseases: Upregulated Expression of TLR2 in Terminal Ileum of Patients With Ulcerative Colitis
J. Histochem. Cytochem., March 1, 2008; 56(3): 267 - 274.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Wehkamp, G. Wang, I. Kubler, S. Nuding, A. Gregorieff, A. Schnabel, R. J. Kays, K. Fellermann, O. Burk, M. Schwab, et al.
The Paneth Cell {alpha}-Defensin Deficiency of Ileal Crohn's Disease Is Linked to Wnt/Tcf-4
J. Immunol., September 1, 2007; 179(5): 3109 - 3118.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Bamias, A. Okazawa, J. Rivera-Nieves, K. O. Arseneau, S. A. De La Rue, T. T. Pizarro, and F. Cominelli
Commensal Bacteria Exacerbate Intestinal Inflammation but Are Not Essential for the Development of Murine Ileitis
J. Immunol., February 1, 2007; 178(3): 1809 - 1818.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Wehkamp, N. H. Salzman, E. Porter, S. Nuding, M. Weichenthal, R. E. Petras, B. Shen, E. Schaeffeler, M. Schwab, R. Linzmeier, et al.
Reduced Paneth cell {alpha}-defensins in ileal Crohn's disease
PNAS, December 13, 2005; 102(50): 18129 - 18134.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. Wehkamp, M. Schmid, K. Fellermann, and E. F. Stange
Defensin deficiency, intestinal microbes, and the clinical phenotypes of Crohn's disease
J. Leukoc. Biol., April 1, 2005; 77(4): 460 - 465.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. A. Ruiz, A. Shkoda, S. C. Kim, R. B. Sartor, and D. Haller
IL-10 Gene-Deficient Mice Lack TGF-{beta}/Smad Signaling and Fail to Inhibit Proinflammatory Gene Expression in Intestinal Epithelial Cells after the Colonization with Colitogenic Enterococcus faecalis
J. Immunol., March 1, 2005; 174(5): 2990 - 2999.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. C. I. Singh, S. M. Cruickshank, D. J. Newton, L. Wakenshaw, A. Graham, J. Lan, J. P. A. Lodge, P. J. Felsburg, and S. R. Carding
Toll-like receptor-mediated responses of primary intestinal epithelial cells during the development of colitis
Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G514 - G524.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
J Wehkamp, J Harder, M Weichenthal, M Schwab, E Schaffeler, M Schlee, K R Herrlinger, A Stallmach, F Noack, P Fritz, et al.
NOD2 (CARD15) mutations in Crohn's disease are associated with diminished mucosal {alpha}-defensin expression
Gut, November 1, 2004; 53(11): 1658 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
D Franchimont, S Vermeire, H El Housni, M Pierik, K Van Steen, T Gustot, E Quertinmont, M Abramowicz, A Van Gossum, J Deviere, et al.
Deficient host-bacteria interactions in inflammatory bowel disease? The toll-like receptor (TLR)-4 Asp299gly polymorphism is associated with Crohn's disease and ulcerative colitis
Gut, July 1, 2004; 53(7): 987 - 992.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
I. Colmegna, R. Cuchacovich, and L. R. Espinoza
HLA-B27-Associated Reactive Arthritis: Pathogenetic and Clinical Considerations
Clin. Microbiol. Rev., April 1, 2004; 17(2): 348 - 369.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
C P Tamboli, C Neut, P Desreumaux, and J F Colombel
Dysbiosis in inflammatory bowel disease
Gut, January 1, 2004; 53(1): 1 - 4.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Sestak, C. K. Merritt, J. Borda, E. Saylor, S. R. Schwamberger, F. Cogswell, E. S. Didier, P. J. Didier, G. Plauche, R. P. Bohm, et al.
Infectious Agent and Immune Response Characteristics of Chronic Enterocolitis in Captive Rhesus Macaques
Infect. Immun., July 1, 2003; 71(7): 4079 - 4086.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
S. Stebbings, K. Munro, M. A. Simon, G. Tannock, J. Highton, H. Harmsen, G. Welling, P. Seksik, J. Dore, G. Grame, et al.
Comparison of the faecal microflora of patients with ankylosing spondylitis and controls using molecular methods of analysis
Rheumatology, December 1, 2002; 41(12): 1395 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. Wei, H. Dalwadi, L. K. Gordon, C. Landers, D. Bruckner, S. R. Targan, and J. Braun
Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody
Infect. Immun., October 1, 2001; 69(10): 6044 - 6054.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Dohi, P. D. Rennert, K. Fujihashi, H. Kiyono, Y. Shirai, Y. I. Kawamura, J. L. Browning, and J. R. McGhee
Elimination of Colonic Patches with Lymphotoxin {beta} Receptor-Ig Prevents Th2 Cell-Type Colitis
J. Immunol., September 1, 2001; 167(5): 2781 - 2790.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
M. CAMPIERI and P. GIONCHETTI
Bacteria as the cause of ulcerative colitis
Gut, January 1, 2001; 48(1): 132 - 135.
[Full Text] [PDF]

Home page
 Infect. Immun.Home page
O. Cohavy, D. Bruckner, L. K. Gordon, R. Misra, B. Wei, M. E. Eggena, S. R. Targan, and J. Braun
Colonic Bacteria Express an Ulcerative Colitis pANCA-Related Protein Epitope
Infect. Immun., March 1, 2000; 68(3): 1542 - 1548.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER
Terms and conditions relating to subscriptions purchased online  ¦  Website terms and conditions  ¦  Privacy policy
Copyright © 1999 BMJ Publishing Group Ltd & British Society of Gastroenterology