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a Department of
Medicine, Division of Gastroenterology, St Luke's-Roosevelt Hospital
Centre, New York, USA, b Department of Pathology, St Luke's-Roosevelt
Hospital Centre, New York, USA, c Departments of Medicine, Anatomy, and Cell
Biology, College of Physicians and Surgeons, Columbia University, New
York, USA, d Howard
Hughes Medical Institute, Laboratory of Cell Biology, The Rockefeller
University, New York, USA
Correspondence to: Dr S F Moss, Division of Gastroenterology, Department of Medicine, St Luke's-Roosevelt Hospital Centre, S&R 12, 1111 Amsterdam Ave, New York, NY 10025, USA.
Accepted for publication 2 June 1999
BACKGROUND
Altered expression of
lamins A/C and B1, constituent proteins of the nuclear lamina, may
occur during differentiation and has also been reported in primary lung cancer.
AIMSTo examine the expression of
these proteins in gastrointestinal neoplasms.
PATIENTSArchival human paraffin
wax blocks and frozen tissue from patients undergoing surgical
resection or endoscopic biopsy.
METHODSImmunohistochemistry and
western blotting using polyclonal antisera against A type lamins and
lamin B1.
RESULTSThe expression of lamin A/C
was reduced and was frequently undetectable by immunohistochemistry in
all primary colon carcinomas and adenomas, and in 7/8 primary gastric
cancers. Lamin B1 expression was reduced in all colon cancers, 16/18
colonic adenomas, and 6/8 gastric cancers. Aberrant, cytoplasmic
labelling with both antibodies occurred in some colonic cancers and
around one third of colonic adenomas. Cytoplasmic lamin A/C expression
was detected in 3/8 gastric cancers. Lamin expression was reduced in
gastric dysplasia, but not intestinal metaplasia, atrophy, or chronic gastritis. Lamin expression was low in carcinomas of oesophagus, prostate, breast, and uterus, but not pancreas.
CONCLUSIONSReduced expression of
nuclear lamins, sometimes together with aberrant, cytoplasmic
immunoreactivity is common in gastrointestinal neoplasms. Altered lamin
expression may be a biomarker of malignancy in the gastrointestinal tract.
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