Gut

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER


[Advanced]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this link to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Add article to my folders
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kidd, M
Right arrow Articles by Louw, J A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kidd, M
Right arrow Articles by Louw, J A
Gut 2001;49:11-17 ( July )

Article

Conservation of the cag pathogenicity island is associated with vacA alleles and gastroduodenal disease in South African Helicobacter pylori isolates M Kidda, A J Lastovicab, J C Athertonc, J A Louwa

a GI Clinic and Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa, b Department of Medical Microbiology, University of Cape Town and Red Cross Hospital, Cape Town, South Africa, c Division of Gastroenterology and Institute of Infections and Immunity, University of Nottingham, Nottingham, UK

Correspondence to: Dr JA Louw, GI Clinic, E23, Groote Schuur Hospital, Observatory, 7925, Cape Town, South Africa. jalouw{at}curie.uct.ac.za.

Accepted for publication 17 January 2001

BACKGROUND---The development of clinically significant disease in South Africa is associated with the vacuolating cytotoxin gene (vacA) s1 genotype but not with the presence of the cytotoxin associated gene cagA. cagA occurs in >95% of South African isolates and is a variable marker for the entire cag pathogenicity island (PAI).
AIM---To characterise the cagPAI in South African isolates and to investigate if structural variants of this multigene locus were associated with variations in vacA status and clinical outcome.
PATIENTS AND METHODS---We studied 109 Helicobacter pylori strains (36 from patients with peptic ulceration, 26 with gastric adenocarcinoma, and 47 with no pathology other than gastritis) for differences in selected genes of the cagPAI and alleles of vacA by polymerase chain reaction.
RESULTS---All strains were cagA+. Sixty five (60%) strains had an intact contiguous cagPAI; 78% of peptic ulcer isolates, 73% of gastric adenocarcinoma isolates, but only 40% of gastritis alone isolates (p< 0.01). The entire cagII region was undetectable in 23% of gastritis alone isolates but in only 8% of peptic ulceration isolates (p<0.05). The vacA signal sequence and mid region demonstrated a strong relationship between the virulence associated vacA s1 (p<0.005) and vacA m1 (p=0.05) alleles and an intact cagPAI.
CONCLUSION---Although a complete cagPAI was a feature of most infected individuals, deletions in the 5' region of this genetic locus were associated with gastritis alone and with the non-cytotoxic s2/m2 vacA genotype.


Keywords: cagA; gastric cancer; Helicobacter pylori; pathogenicity; peptic ulcer; vacuolating cytotoxin

© 2001 by Gut


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
A. Reyes-Leon, J. C. Atherton, R. H. Argent, J. L. Puente, and J. Torres
Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene
Infect. Immun., July 1, 2007; 75(7): 3445 - 3454.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
R. Mattar, S. B. Marques, M. d. S. Monteiro, A. F. dos Santos, K. Iriya, and F. J. Carrilho
Helicobacter pylori cag pathogenicity island genes: clinical relevance for peptic ulcer disease development in Brazil
J. Med. Microbiol., January 1, 2007; 56(1): 9 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. H. Argent, Y. Zhang, and J. C. Atherton
Simple Method for Determination of the Number of Helicobacter pylori CagA Variable-Region EPIYA Tyrosine Phosphorylation Motifs by PCR
J. Clin. Microbiol., February 1, 2005; 43(2): 791 - 795.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. Kauser, A. A. Khan, M. A. Hussain, I. M. Carroll, N. Ahmad, S. Tiwari, Y. Shouche, B. Das, M. Alam, S. M. Ali, et al.
The cag Pathogenicity Island of Helicobacter pylori Is Disrupted in the Majority of Patient Isolates from Different Human Populations
J. Clin. Microbiol., November 1, 2004; 42(11): 5302 - 5308.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
M. Ong, K. Moozar, and L. B. Cohen
Octreotide in Bronchobiliary Fistula Management
Ann. Thorac. Surg., October 1, 2004; 78(4): 1512 - 1513.
[Full Text] [PDF]

Home page
 Postgrad. Med. J.Home page
A Ewart, L Harrison, B Joyner, and A Safe
Providing treatment for hepatitis C in an Australian district centre
Postgrad. Med. J., March 1, 2004; 80(941): 180 - 182.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Nilsson, A. Sillen, L. Eriksson, M.-L. Strand, H. Enroth, S. Normark, P. Falk, and L. Engstrand
Correlation between cag Pathogenicity Island Composition and Helicobacter pylori-Associated Gastroduodenal Disease
Infect. Immun., November 1, 2003; 71(11): 6573 - 6581.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER
Terms and conditions relating to subscriptions purchased online  ¦  Website terms and conditions  ¦  Privacy policy
Copyright © 2001 BMJ Publishing Group Ltd & British Society of Gastroenterology