| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
|
|
|
|
|||||||||||||
|
|
|||||||||||||||
a Laboratoire de
Biochimie Médicale, Faculté de Médecine, 44035 Nantes, France, b INSERM U539,
Faculté de Médecine, 44035 Nantes, France
Correspondence to: Dr MG Denis, Laboratoire de Biochimie Spécialisée, Institut de Biologie, 9 quai Moncousu, F-44035 Nantes Cedex, France. mdenis{at}sante.univ-nantes.fr
Accepted for publication 29 January 2001
BACKGROUND AND
AIMS
The Tage4 gene
(tumour associated glycoprotein E4) is overexpressed in rat colon
tumours and Min mouse intestinal adenomas. The rat Tage4 protein has
approximately 40% identity with human CD155, a member of the
immunoglobulin superfamily coding for a transmembrane protein capable
of serving as an entry receptor for poliovirus, porcine
pseudorabies virus, and bovine herpesvirus 1. Analysis of the rat
Tage4 gene has revealed structural and functional similarities with the human CD155
gene. We therefore investigated expression of the
CD155 gene in human colorectal carcinomas.
METHODSOverall CD155
expression was assessed by semiquantitative reverse
transcription-polymerase chain reaction (RT-PCR) and
immunohistochemical analysis using tissue specimens from patients with
colorectal adenomas and adenocarcinomas. We also used a qualitative
RT-PCR assay to determine relative expression of different splicing
variants in each sample.
RESULTSmRNA levels of
CD155 were increased in six of six colorectal cancer tissues compared
with the tumour free colon mucosa. Immunohistochemical analysis
revealed an increased level of CD155 protein in 12 of 12 samples. The
qualitative RT-PCR assay revealed that relative expression of the
different CD155 variant transcripts was similar in the different normal
and cancer samples tested, indicating that this overexpression is not
associated with a particular mRNA variant generated by alternative
splicing of the CD155 gene.
CONCLUSIONWe have
shown for the first time that the CD155 gene
is overexpressed in colorectal carcinoma and that this overexpression begins at an early stage in tumorigenesis and continues to late stages.
This article has been cited by other articles:
|
|
 |
|
  A. Iguchi-Manaka, H. Kai, Y. Yamashita, K. Shibata, S. Tahara-Hanaoka, S.-i. Honda, T. Yasui, H. Kikutani, K. Shibuya, and A. Shibuya Accelerated tumor growth in mice deficient in DNAM-1 receptor J. Exp. Med., November 24, 2008; (2008) jem.20081611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takahashi, Y. Rikitake, Y. Nagamatsu, T. Hara, W. Ikeda, K.-i. Hirata, and Y. Takai Sequential activation of Rap1 and Rac1 small G proteins by PDGF locally at leading edges of NIH3T3 cells. Genes Cells, June 1, 2008; 13(6): 549 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Amano, W. Ikeda, S. Kawano, M. Kajita, Y. Tamaru, N. Inoue, Y. Minami, A. Yamada, and Y. Takai Interaction and localization of Necl-5 and PDGF receptor beta at the leading edges of moving NIH3T3 cells: Implications for directional cell movement. Genes Cells, March 1, 2008; 13(3): 269 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Minami, W. Ikeda, M. Kajita, T. Fujito, H. Amano, Y. Tamaru, K. Kuramitsu, Y. Sakamoto, M. Monden, and Y. Takai Necl-5/Poliovirus Receptor Interacts in cis with Integrin {alpha}Vbeta3 and Regulates Its Clustering and Focal Complex Formation J. Biol. Chem., June 22, 2007; 282(25): 18481 - 18496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kajita, W. Ikeda, Y. Tamaru, and Y. Takai Regulation of platelet-derived growth factor-induced Ras signaling by poliovirus receptor Necl-5 and negative growth regulator Sprouty2 Genes Cells, March 1, 2007; 12(3): 345 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bachelet, A. Munitz, D. Mankutad, and F. Levi-Schaffer Mast Cell Costimulation by CD226/CD112 (DNAM-1/Nectin-2): A NOVEL INTERFACE IN THE ALLERGIC PROCESS J. Biol. Chem., September 15, 2006; 281(37): 27190 - 27196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tahara-Hanaoka, K. Shibuya, H. Kai, A. Miyamoto, Y. Morikawa, N. Ohkochi, S.-i. Honda, and A. Shibuya Tumor rejection by the poliovirus receptor family ligands of the DNAM-1 (CD226) receptor Blood, February 15, 2006; 107(4): 1491 - 1496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Sloan, J. K. Stewart, A. F. Treloar, R. T. Matthews, and D. G. Jay CD155/PVR Enhances Glioma Cell Dispersal by Regulating Adhesion Signaling and Focal Adhesion Dynamics Cancer Res., December 1, 2005; 65(23): 10930 - 10937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fujito, W. Ikeda, S. Kakunaga, Y. Minami, M. Kajita, Y. Sakamoto, M. Monden, and Y. Takai Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3 J. Cell Biol., October 10, 2005; 171(1): 165 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Boles, W. Barchet, T. Diacovo, M. Cella, and M. Colonna The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM Blood, August 1, 2005; 106(3): 779 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, K. Irie, T. Ooshio, W. Ikeda, and Y. Takai Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions Genes Cells, September 1, 2004; 9(9): 791 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kakunaga, W. Ikeda, T. Shingai, T. Fujito, A. Yamada, Y. Minami, T. Imai, and Y. Takai Enhancement of Serum- and Platelet-derived Growth Factor-induced Cell Proliferation by Necl-5/Tage4/Poliovirus Receptor/CD155 through the Ras-Raf-MEK-ERK Signaling J. Biol. Chem., August 27, 2004; 279(35): 36419 - 36425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ochiai, S. A. Moore, G. E. Archer, T. Okamura, T. A. Chewning, J. R. Marks, J. H. Sampson, and M. Gromeier Treatment of Intracerebral Neoplasia and Neoplastic Meningitis with Regional Delivery of Oncolytic Recombinant Poliovirus Clin. Cancer Res., July 15, 2004; 10(14): 4831 - 4838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Merrill, G. Bernhardt, J. H. Sampson, C. J. Wikstrand, D. D. Bigner, and M. Gromeier Poliovirus receptor CD155-targeted oncolysis of glioma Neuro-oncol, July 1, 2004; 6(3): 208 - 217. [Abstract] [PDF]
|
|
|
|
|
|
W. Ikeda, S. Kakunaga, K. Takekuni, T. Shingai, K. Satoh, K. Morimoto, M. Takeuchi, T. Imai, and Y. Takai Nectin-like Molecule-5/Tage4 Enhances Cell Migration in an Integrin-dependent, Nectin-3-independent Manner J. Biol. Chem., April 23, 2004; 279(17): 18015 - 18025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fuchs, M. Cella, E. Giurisato, A. S. Shaw, and M. Colonna Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155) J. Immunol., April 1, 2004; 172(7): 3994 - 3998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mueller and E. Wimmer Recruitment of Nectin-3 to Cell-Cell Junctions through trans-Heterophilic Interaction with CD155, a Vitronectin and Poliovirus Receptor That Localizes to {alpha}v{beta}3 Integrin-containing Membrane Microdomains J. Biol. Chem., August 15, 2003; 278(33): 31251 - 31260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-S. Gosselin, Y. Simonin, F. Guivel-Benhassine, V. Rincheval, J.-L. Vayssiere, B. Mignotte, F. Colbere-Garapin, T. Couderc, and B. Blondel Poliovirus-Induced Apoptosis Is Reduced in Cells Expressing a Mutant CD155 Selected during Persistent Poliovirus Infection in Neuroblastoma Cells J. Virol., December 6, 2002; 77(1): 790 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Solecki, M. Gromeier, S. Mueller, G. Bernhardt, and E. Wimmer Expression of the Human Poliovirus Receptor/CD155 Gene Is Activated by Sonic Hedgehog J. Biol. Chem., July 5, 2002; 277(28): 25697 - 25702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Ansardi, D. C. Porter, C. A. Jackson, G. Y. Gillespie, and C. D. Morrow RNA Replicons Derived from Poliovirus Are Directly Oncolytic for Human Tumor Cells of Diverse Origins Cancer Res., December 1, 2001; 61(23): 8470 - 8479. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
