HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER		Author Keyword(s) Vol Page [Advanced]

This Article Full Text Full Text (PDF) Correction (v54,p442) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this link to a friend Similar articles in this journal Similar articles in PubMed Add article to my folders Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sheu, B-S Articles by Wu, J-J Search for Related Content PubMed PubMed Citation Articles by Sheu, B-S Articles by Wu, J-J

Host gastric Lewis expression determines the bacterial density of Helicobacter pylori in babA2 genopositive infection

¹ Institute of Clinical Medicine, and Department of Internal Medicine, National Cheng Kung University, Medical College, Tainan, Taiwan
² Institute of Basic Medical Sciences, National Cheng Kung University, Medical College, Tainan, Taiwan
³ Department of Pathology, National Cheng Kung University, Medical College, Tainan, Taiwan
⁴ Department of Medical Technology, and Institute of Basic Medical Sciences, National Cheng Kung University, Medical College, Tainan, Taiwan

Correspondence to:
Correspondence to:
Professor J-J Wu, Department of Medical Technology, National Cheng Kung University, Medical College, No 1, University Road, Tainan, Taiwan 701;
jjwu{at}mail.ncku.edu.tw

ABSTRACT
Background and aims: We tested if host gastric Lewis antigens and the babA2 genotype of Helicobacter pylori correlated with clinicohistological outcome.

Methods: We enrolled 188 dyspeptic patients (45 with duodenal ulcer, 45 with gastric ulcer, and 98 with chronic gastritis) with H pylori infection, proved by culture and gastric histology, reviewed by the updated Sydney system. Gastric expression of Lewis (Le) antigens Le^a, Le^b, Le^x, and Le^y was determined immunochemically to determine intensity (range 0–3). The corresponding 188 H pylori isolates were screened for babA2 genotype by polymerase chain reaction.

Results: All H pylori isolates had a positive babA2 genotype. We identified Le^a in 33.5%, Le^b in 72.9%, Le^x in 86.2%, and Le^y in 97.4% of biopsies from these 188 patients. Patients who expressed Le^b had a higher H pylori density than those who did not express Le^b (p<0.001). Among 139 patients who expressed Le^b, H pylori density increased with a higher Le^b intensity (p<0.05). Gastric atrophy decreased with Le^b intensity and thus resulted in lower H pylori density in the antrum (p<0.05). For the 49 patients without gastric Le^b expression, H pylori density was positively related with Le^x and Le^a expression (p<0.05).

Conclusions: Taiwanese H pylori isolates are 100% babA2 genopositive. Gastric Le^b as well as Le^x intensity may be major determinants of H pylori density. While lacking gastric Le^b expression, Le^x and Le^a were closely related to H pylori colonisation.

Keywords: Helicobacter pylori; babA2; Lewis antigen expression; gastric histology

Abbreviations: Le, Lewis; Lewis-N, total Lewis number; BabA, blood group antigen binding adhesin; babA2, blood group associated binding gene; PCR, polymerase chain reaction; TLI, total gastric Lewis antigen expression intensity; HPD, Helicobacter pylori density; IM, intestinal metaplasia; CIS, chronic inflammatory score; PBS, phosphate buffered saline

This article has been cited by other articles:

				B.-S. Sheu and J.-J. Wu Type 1 and 2 Lewis antigens of Helicobacter pylori - a potential marker of the human geographical distribution J. Med. Microbiol., May 1, 2008; 57(5): 543 - 544. [Full Text] [PDF]

				E. Altman, H. Fernandez, V. Chandan, B. A. Harrison, M. W. Schuster, L. O. Rademacher, and C. Toledo Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide J. Med. Microbiol., May 1, 2008; 57(5): 585 - 591. [Abstract] [Full Text] [PDF]

				S.-M. Sheu, B.-S. Sheu, H.-B. Yang, H.-Y. Lei, and J.-J. Wu Anti-Lewis X Antibody Promotes Helicobacter pylori Adhesion to Gastric Epithelial Cells Infect. Immun., June 1, 2007; 75(6): 2661 - 2667. [Abstract] [Full Text] [PDF]

				B.-S. Sheu, H.-C. Cheng, A.-W. Kao, S.-T. Wang, Y.-J. Yang, H.-B. Yang, and J.-J. Wu Pretreatment with Lactobacillus- and Bifidobacterium-containing yogurt can improve the efficacy of quadruple therapy in eradicating residual Helicobacter pylori infection after failed triple therapy. Am. J. Clinical Nutrition, April 1, 2006; 83(4): 864 - 869. [Abstract] [Full Text] [PDF]

				Y. J. Chang, M. S. Wu, J. T. Lin, B. S. Sheu, T. Muta, H. Inoue, and C.-C. Chen Induction of Cyclooxygenase-2 Overexpression in Human Gastric Epithelial Cells by Helicobacter pylori Involves TLR2/TLR9 and c-Src-Dependent Nuclear Factor-{kappa}B Activation Mol. Pharmacol., December 1, 2004; 66(6): 1465 - 1477. [Abstract] [Full Text] [PDF]

				A. Backstrom, C. Lundberg, D. Kersulyte, D. E. Berg, T. Boren, and A. Arnqvist From The Cover: Metastability of Helicobacter pylori bab adhesin genes and dynamics in Lewis b antigen binding PNAS, November 30, 2004; 101(48): 16923 - 16928. [Abstract] [Full Text] [PDF]