| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
|
|
|
|
|||||||||||||
|
|
|||||||||||||||
INTESTINE |
1 Laboratoire de Biologie de la Nutrition, EA 2498, Faculté de Pharmacie-Université Paris 5, Paris, France
2 Laboratoire de Biologie de la Nutrition, EA 2498, Faculté de Pharmacie-Université Paris 5, Paris, France, and Laboratoire de Biochimie, Hôtel-Dieu, AP-HP, Paris, France
Correspondence to:
Correspondence to:
Dr S Osowska
Laboratoire de Biologie de la Nutrition, Faculté de Pharmacie-Université Paris 5, 4 Avenue de l’Observatoire, 75270 Paris Cedex 06, France; moinard{at}pharmacie.univ-paris5.fr
ABSTRACT
Objective: Arginine supplementation seems logical in situations where this amino acid becomes essential, for example after massive intestinal resection. Arginine is taken up and metabolised by the liver to a large extent and its supplementation is potentially unsafe. Citrulline is not captured by the liver and passes freely to the kidneys where it is metabolised to arginine, and so is a good candidate to generate arginine and thereby improve nutritional status.
Methods: Twenty four rats were assigned to four groups: citrulline, arginine, control, and sham. The sham group underwent transection and the three other groups resection of 80% of the small intestine. All rats were fed by enteral nutrition and its composition was as follows: supplementation with citrulline in the citrulline group, supplementation with arginine in the arginine group, and standard polymeric enteral nutrition in the control and sham groups. All groups received isonitrogenous nutrition and citrulline and arginine intakes were equimolar in their respective groups. After 10 days, the rats were sacrificed.
Results: Arginine concentration was higher (p<0.05) in plasma and muscle in the citrulline group than in the three other groups. Plasma levels of arginine were 110 (12), 79 (7), 167 (22), and 228 (13) µmol/l in the sham, control, arginine, and citrulline groups respectively. Arginine concentrations in the gastrocnemius were: 0.15 (0.02), 0.16 (0.02), 0.40 (0.05), and 0.94 (0.20) µmol/g, respectively. Citrulline preserved nitrogen balance in resected rats but not in arginine supplemented rats (mean J10: 2.27 (0.29), 1.67 (0.15), 1.98 (0.29), and 2.43 (0.41) g/24 hours in the sham, control, arginine, and citrulline groups, respectively).
Conclusion: Supplementing the diet with citrulline is a very efficient means of increasing arginine levels and improving nitrogen balance after massive intestinal resection. The results of this study form a strong rationale for citrulline supplementation in these patients.
Abbreviations: EDL, extensor digitorum longus; SBS, short bowel syndrome
Keywords: short bowel syndrome; intestinal adaptation; arginine; citrulline
This article has been cited by other articles:
|
|
 |
|
  A. Barbul Proline Precursors to Sustain Mammalian Collagen Synthesis J. Nutr., October 1, 2008; 138(10): 2021S - 2024S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Bertolo and D. G. Burrin Comparative Aspects of Tissue Glutamine and Proline Metabolism J. Nutr., October 1, 2008; 138(10): 2032S - 2039S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Raynaud-Simon, M. Kuhn, J. Moulis, J. Marc, L. Cynober, and C. Loi Tolerance and Efficacy of a New Enteral Formula Specifically Designed for Elderly Persons: An Experimental Study in the Aged Rat J. Gerontol. A Biol. Sci. Med. Sci., July 1, 2008; 63(7): 669 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rouge, C. Des Robert, A. Robins, O. Le Bacquer, C. Volteau, M.-F. De La Cochetiere, and D. Darmaun Manipulation of citrulline availability in humans Am J Physiol Gastrointest Liver Physiol, November 1, 2007; 293(5): G1061 - G1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Moinard and L. Cynober Citrulline: A New Player in the Control of Nitrogen Homeostasis J. Nutr., June 1, 2007; 137(6): 1621S - 1625S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. van de Poll, M. P. Siroen, P. A. van Leeuwen, P. B Soeters, G. C Melis, P. G Boelens, N. E. Deutz, and C. H. Dejong Interorgan amino acid exchange in humans: consequences for arginine and citrulline metabolism Am. J. Clinical Nutrition, January 1, 2007; 85(1): 167 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Osowska, T. Duchemann, S. Walrand, A. Paillard, Y. Boirie, L. Cynober, and C. Moinard Citrulline modulates muscle protein metabolism in old malnourished rats Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E582 - E586. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
