| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
|
|
|
|
|||||||||||||
|
|
|||||||||||||||
HELICOBACTER PYLORI |
1 Department of Internal Medicine II and Gastroenterology, Technical University of Munich, Munich, Germany
2 Institute of Medical Microbiology and Immunology, Technical University of Munich, Munich, Germany
Correspondence to:
Correspondence to:
Professor C Prinz
Klinikum Rechts der Isar der Technischen Universität München, II Medizinische Klinik, Ismaningerstraße 22, 81675 München, Germany; christian.prinz{at}lrz.tum.de
Background and aims: Recent studies linked cytokine gene polymorphisms to H pylori related gastric cancer development. The current study evaluated the role of cytokine gene polymorphisms for mucosal cytokine expression, the gastric inflammatory response, and bacterial colonisation during H pylori infection.
Patients and methods: In 207 H pylori infected patients with chronic gastritis, polymorphisms at different loci of the interleukin (IL)-10, IL-1B, IL-1 receptor antagonist (IL-1RN), tumour necrosis factor (TNF)-A, and interferon (IFN)-G genes were genotyped by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) analysis, and allelic discriminating TaqMan PCR. Mucosal cytokine mRNA copy numbers were determined by real time quantitative PCR. Presence of bacterial virulence factors was investigated by cagA, vacAs1/2, and babA2 PCR. Biopsies were assessed with regard to the degrees of granulocytic/lymphocytic infiltration and the presence of intestinal metaplasia (IM) and atrophic gastritis (AG).
Results: Proinflammatory IL-1 polymorphisms (IL-1RN*2+/IL-1B–511T/–31C+) were associated with increased IL-1ß expression, more severe degrees of inflammation, and an increased prevalence of IM and AG. Carriers of the IL-10–1082G/–819C/–592C alleles (GCC haplotype) had higher mucosal IL-10 mRNA levels than ATA haplotype carriers and were associated with colonisation by more virulent cagA+, vacAs1+, and babA2+ H pylori strains. The TNF-A–307(G/A) and IFN-G+874(A/T) polymorphisms did not influence mucosal cytokine expression or the inflammatory response to H pylori.
Conclusions: Cytokine gene polymorphisms influence mucosal cytokine expression, gastric inflammation, and the long term development of precancerous lesions in H pylori infection. Host polymorphisms are associated with certain bacterial strain types, suggesting host specific colonisation or adaptation. These findings contribute to the understanding of the complex interplay between host and bacterial factors involved in the development of gastric pathology.
Abbreviations: cagPAI, cag pathogenicity island; VacA, vacuolating cytotoxin; BabA, blood group antigen binding adhesin; IFN-
, interferon ; TNF-
, tumour necrosis factor ; IL, interleukin; IL-1RN, IL-1 receptor antagonist; SNP, single nucleotide polymorphism; PBMC, peripheral blood mononuclear cells; VNTR, variable number of tandem repeat region; IM, intestinal metaplasia; AG, atrophic gastritis; PCR, polymerase chain reaction; RFLP, restriction fragment length polymorphism; OR, odds ratio; GAPDH, glyceraldehyde-3-phosphate dehydrogenase
Keywords: Helicobacter pylori; cytokines; polymorphisms; cagA; vacA; babA
Relevant Article
Gut 2004 53: 1059.
This article has been cited by other articles:
|
|
 |
|
  R. Mahajan, E. M. El-Omar, J. Lissowska, P. Grillo, C. S. Rabkin, A. Baccarelli, M. Yeager, L. H. Sobin, W. Zatonski, S. J. Channock, et al. Genetic Variants in T Helper Cell Type 1, 2 and 3 Pathways and Gastric Cancer Risk in a Polish Population Jpn. J. Clin. Oncol., September 1, 2008; 38(9): 626 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.M.G. Moons, J.G. Kusters, J.H.M. van Delft, E.J. Kuipers, R. Gottschalk, H. Geldof, W.A. Bode, J. Stoof, A.H.M. van Vliet, H.B. Ketelslegers, et al. A pro-inflammatory genotype predisposes to Barrett's esophagus Carcinogenesis, May 1, 2008; 29(5): 926 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zabaleta, H.-Y. Lin, R. A. Sierra, M.C. Hall, P. E. Clark, O. A. Sartor, J. J. Hu, and A. C. Ochoa Interactions of cytokine gene polymorphisms in prostate cancer risk Carcinogenesis, March 1, 2008; 29(3): 573 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Ragouilliaux, S. E. Keeney, H. K. Hawkins, and J. L. Rowen Maternal Factors in Extremely Low Birth Weight Infants Who Develop Spontaneous Intestinal Perforation Pediatrics, December 1, 2007; 120(6): e1458 - e1464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers Pathogenesis of Helicobacter pylori Infection Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ruggiero, F. Tombola, G. Rossi, L. Pancotto, L. Lauretti, G. Del Giudice, and M. Zoratti Polyphenols Reduce Gastritis Induced by Helicobacter pylori Infection or VacA Toxin Administration in Mice. Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2550 - 2552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kerk,, M. Dordelmann, D. B. Bartels, M.-J. Brinkhaus, C. E. L. Dammann, T. Dork, and O. Dammann MUltiplex Measurement of Cytokine/Receptor Gene Polymorphisms and interaction Between Interleukin-10 (-1082) Genotype and Chorioamnionitis in Extreme Preterm Delivery Reproductive Sciences, July 1, 2006; 13(5): 350 - 356. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Rad, R. M. Schmid, and C. Prinz Helicobacter pylori, iron deficiency, and gastric autoimmunity Blood, June 15, 2006; 107(12): 4969 - 4970. [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Fritz, T. Slavik, W. Delport, B. Olivier, and S. W. van der Merwe Incidence of Helicobacter felis and the Effect of Coinfection with Helicobacter pylori on the Gastric Mucosa in the African Population. J. Clin. Microbiol., May 1, 2006; 44(5): 1692 - 1696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S Kornman Interleukin 1 genetics, inflammatory mechanisms, and nutrigenetic opportunities to modulate diseases of aging Am. J. Clinical Nutrition, February 1, 2006; 83(2): 475S - 483S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Ando, E M El-Omar, Y Goto, K Nobata, O Watanabe, O Maeda, K Ishiguro, M Minami, N Hamajima, and H Goto Interleukin 1B proinflammatory genotypes protect against gastro-oesophageal reflux disease through induction of corpus atrophy Gut, February 1, 2006; 55(2): 158 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. I. Perez-Perez, E. Garza-Gonzalez, C. Portal, and A. Z. Olivares Role of Cytokine Polymorphisms in the Risk of Distal Gastric Cancer Development Cancer Epidemiol. Biomarkers Prev., August 1, 2005; 14(8): 1869 - 1873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Whary, N. Sundina, L. E. Bravo, P. Correa, F. Quinones, F. Caro, and J. G. Fox Intestinal Helminthiasis in Colombian Children Promotes a Th2 Response to Helicobacter pylori: Possible Implications for Gastric Carcinogenesis Cancer Epidemiol. Biomarkers Prev., June 1, 2005; 14(6): 1464 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Hellmig, J Hampe, U R Folsch, and S Schreiber Role of IL-10 promoter haplotypes in Helicobacter pylori associated gastric inflammation Gut, June 1, 2005; 54(6): 888 - 888. [Full Text] [PDF]
|
|
|
|
 |
|
 M Kudo, O Gutierrez, H M T El-Zimaity, H Cardona, Z Z Nurgalieva, J Wu, and D Y Graham CagA in Barrett's oesophagus in Colombia, a country with a high prevalence of gastric cancer J. Clin. Pathol., March 1, 2005; 58(3): 259 - 262. [Abstract] [Full Text] [PDF]
|
|
Read all eLetters
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
