Target gene expression | n | Tissues | p Value* | T/NT ratio | |
Non-tumoural | Tumoural | ||||
IL8 | (45) | 0.3 (0.1–0.7) | 7.5 (3.4–12.2) | <0.0001 | |
MSS | (31) | 0.2 (0.1–0.7) | 7.5 (3.4–11.0) | <0.0001 | 35.5 |
MSI-H | (14) | 0.4 (0.1–0.9) | 9.8 (3.0–16.5) | 0.001 | 26.0 |
p Value† | 0.79 | ||||
CXCR1 | (45) | 0.2 (0.1–0.4) | 0.6 (0.3–1.3) | 0.004 | |
MSS | (31) | 0.2 (0.1–0.4) | 0.5 (0.2–0.7) | ||
MSI-H | (14) | 0.2 (0.001–0.8) | 0.9 (0.4–2.9) | ||
p Value† | |||||
IL1β | (45) | 336 (104–1047) | 2143 (686–4729) | <0.0001 | |
MSS | (31) | 330 (73 – 622) | 1852 (489–3392) | <0.0001 | 4.7 |
MSI-H | (14) | 388 (172–1290) | 3844 (686–5756) | 0.01 | 4.5 |
p Value† | 0.79 | ||||
IL6 | (45) | 1014 (297–4837) | 12 281 (5942–23 507) | 0.003 | |
MSS | (31) | 904 (247–4815) | 12 824 (3597–28 160) | 0.002 | 19.6 |
MSI-H | (14) | 1764 (726–11418) | 11 357 (7165–17 411) | 0.36 | 3.6 |
p Value† | 0.08 | ||||
TGFβ | (45) | 1878 (975–2992) | 4488 (2191–6061) | <0.0001 | |
MSS | (31) | 1755 (962–3030) | 3941 (1953–5935) | <0.0001 | 2.2 |
MSI-H | (14) | 2007 (1054–2728) | 5264 (2818–9656) | 0.004 | 2.5 |
p Value† | 0.71 | ||||
TNFα | (45) | 133 (75–269) | 216 (153–390) | 0.22 | |
MSS | (31) | 104 (65–234) | 199 (139–381) | ||
MSI-H | (14) | 192 (130–1359) | 222 (174–492) | ||
p Value† |
Results are expressed as median (first–third quartiles) of the ratio of the target gene to the housekeeping gene mRNA copy numbers.
*p Value of the paired non-parametric comparisons between tumoural and non-tumoural tissues (Wilcoxon signed-rank test).
†p Value of the non-parametric comparisons between MSS and MSI-H phenotypes.
After using the Bonferroni correction for multiple testing, p⩽0.003 (0.05/15) was considered statistically significant and these values are indicated in bold.
CXCR, chemokine receptor; IL, interleukin; MSS, microsatellite stability; MSI-H, high-frequency microsatellite instability; NT, non-tumoural; T, tumoural; TGF, transforming growth factor; TNF, tumour necrosis factor.