Summary
In the external layers of human small and large intestine macrophage-like cells were characterized by immunohistochemical, histochemical and electronmicroscopical methods. Using immunohistochemistry and a number of monoclonal antibodies, the presence and distribution of phenotypic subpopulations of macrophages were evaluated. In all locations macrophage-like cells were identified with antibody EBM11, which recognizes CD68 antigen, C3bi which recognizes CD11b, and partly with an antibody which recognizes protein 150,95 (CD11c). Macrophage-like cells in the external muscle layer were HLA-DR-positive (expressing the MHC class-II antigen), in contrast to macrophage-like cells in the subserosa and submucosa. Macrophage-like cells in the external muscle layer were mostly acid phosphatase-negative, and at the electron-microscopic level they were found to have features of macrophages: primary lysosomes, coated vesicles and pits. However, very few secondary lysosomes were present. Birbeck granules were not observed. It is concluded that in the external muscle layer of human small and large intestine numerous macrophages of a special type are present. It is discussed whether this cell type plays a role in gastrointestinal motility and/or has an immunological function.
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Arizono N, Matsuda S, Hattori T, Kojima Y, Maeda T, Galli SJ (1990) Anatomical variation in mast cell nerve associations in rat small intestine, heart, lung, and skin. Lab Invest 62:626–634
Beller DI, Springer TA, Schreiber RD (1982) Anti-Mac-1 selectively inhibits the mouse and the human type three complement receptor J Exp Med 156:1000–1009
Franklin WA, Mason DY, Pulford K, Falini B, Bliss E, Gatter KC, Stein H, Clarke LC, McGee JO'D (1986) Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest 54:322–335
Harvey J, Jones DB (1991) Review-Human mucosal T-lymphocyte and macrophage subpopulations in normal and inflamed intestine. Clin Exp Allergy 21:549–560
Hinglais N, Kazatchkine MD, Mandet C, Appay MD, Bariety J (1989) Human liver Kupffer cells express CR1, CR3, and CR4 complement receptor antigens. An immunohistochemical study. Lab Invest 61:509–514
Hume DA (1985) Immunohistochemical analysis of murine mononuclear phagocytes that express class II major histocompatibility antigens. Immunobiology 170:381–389
Hume DA, Allan W, Hogan PG, Doe WF (1987) Immunohistochemical characterization of macrophages in human liver and gastrointestinal tract: Expression of CD4, HLA-DR, OKM1, and the mature macrophage marker 25F9 in normal and diseased tissue. J Leukoc Biol 42:474–484
Hynes RO (1987) Integrins: A family of cell surface receptors. Cell 48:549–554
Kelly PMA Bliss E, Morton JA, Burns J, McGee JO'D (1988) Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol 41:510–515
Lojda Z, Grossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New York
Malizia G, Calabrese A, Cottone M, Raimondo M, Trejdosiewicz LK, Smart CJ, Oliva L, Pagliaro L (1991) Expression of leukocyte adhesion molecules by mucosal mononuclear phagocytes in inflammatory bowel disease. Gastroenterology 100:150–159
Mayer L, Eisenhardt D, Salomon P Bauer W, Plous R, Piccinini L (1991) Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease. Gastroenterology 100:3–12
Micklem KJ, Rigney E, Cordell J, Simmons D, Stross P, Turley H, Seed B, Mason DY (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Br J Haematol 73:6–11
Mikkelsen HB, Thuneberg L, Rumessen JJ, Thorball N (1985) Macrophage-like cells in the muscularis externa of mouse small intestine. Anat Rec 213:77–86
Mikkelsen HB, Mirsky R, Jessen KR, Thuneberg L (1988a) Macrophage-like cells in muscularis externa of mouse small intestine: Immunohistochemical localization of F4/80, M1/70, and Ia-antigen. Cell Tissue Res 252:301–306
Mikkelsen HB, Thuneberg L, Wittrup IH (1988b) Selective double staining of intestitial cells of Cajal and macrophage-like cells in small intestine by an improved supravital methylene blue technique combined with FITC-dextran uptake. Anat Embryol (Berl) 178:191–195
Mikkelsen HB, Rumessen IT, Thuneberg L (1990) Prostaglandin h synthase immunoreactivity localized by immunoperoxidase technique (pap) in the small intestine and kidney of rabbit and guinea-pig. Histochemistry 93:363–367
Mikkelsen HB, Rumessen JJ, Qvortrup K (1991) Prostaglandin h synthase immunoreactivity in human gut. An immunohistochemical study. Histochemistry 96:295–299
Myones BL, Dalzell JG, Hogg N, Ross GD (1988) Neutrophil and monocyte cell surface p150,95 has iC3b-receptor (CR4) activity resembling CR3 J Clin Invest 82:640–651
Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79:319–326
Oliver AM, Thomson AW, Sewell HF, Abramovich DR (1988) Major histocompatibility complex (MHC) class II antigen (HLA-DR, DQ, and DP) expression in human fetal endocrine organs and gut. Scand J Immunol 27:731–737
Rumessen JJ, Thuneberg L (1991) Interstitial cells of Cajal in human small intestine. Ultrastructural identification and organization between the main smooth muscle layers. Gastroenterology 100:1417–1431
Rumessen JJ, Mikkelsen HB, Thuneberg L (1992) Ultrastructure of interstitial cells of Cajal associated with deep muscular plexus of human small intestine. Gastroenterology 102:56–68
Selby WS, Poulter LW, Hobbs S, Jewell DP, Janossy G (1983) Heterogeneity of HLA-DR-positive histiocytes in human intestinal lamina propria: a combined histochemical and immunohistological analysis. J Clin Pathol 36:379–384
Spencer J, MacDonald TT, Isaacson PG (1987) Heterogeneity of non-lymphoid cells expressing HLA-D region antigens in human fetal gut. Clin Exp Immunol 67:415–424
Springer TA, Dustin ML, Kishimoto TK, Marlin SD (1987) The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: Cell adhesion receptors of the immune system. Ann Rev Immunol 5:223–252
Thuneberg L (1982) Interstitial cells of Cajal: intestinal pacemaker cells? Adv Anat Embryol Cell Biol 71:1–130
Wilders MM, Drexhage HA, Kokje M, Verspaget HW, Meuwissen SGM (1984) Veiled cells in chronic idiopathic inflammatory bowel disease. Clin Exp Immunol 55:377–387
Wilders MM, Drexhage HA, Sminia T, Mullink H, Weltevreden EF, Plesh BEC, Meuwissen SGM (1985) Veiled cells in the gastrointestinal tract. Acta Chir Scand 525 [Suppl]:93–111
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Mikkelsen, H.B., Rumessen, J.J. Characterization of macrophage-like cells in the external layers of human small and large intestine. Cell Tissue Res 270, 273–279 (1992). https://doi.org/10.1007/BF00328013
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DOI: https://doi.org/10.1007/BF00328013