Summary
A noncholinergic nicotine receptor on human phagocytic leukocytes has been characterized using the binding of3H-(d,l)-nicotine. The average affinity +/-standard deviation of (d,l)-nicotine for the receptor on neutrophils is 36 ± 18 nM (n=6). The binding is saturable with an average of 8.7 x 104 sites per neutrophil. Monocytes and to a lesser extent lymphocytes but not erythrocytes also display specific binding. Bound nicotine is dissociable from the receptor and is not metabolized. Only close structural analogs of nicotine bind to the receptor, which is stereoselective for the (d)-isomer. The receptor can be occupied by (l)-nicotine at concentrations present in the blood of smokers. It is suggested that some of the adverse effects of smoking on leukocyte functions may be mediated by a specific nicotine receptor.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abood, L. G., Lowy, K., Tometsko, A. & MacNeil, M., 1979. Arch. Int. Pharmacodyn Ther. 237: 213–229.
Romano, C. & Goldstein, A., 1980. Science 210(7): 647–650.
Noble, R. C. & Penny, B. P., 1975. Infection and Immunity 12(3): 550–555.
Friedman, G. D., Siegelaub, A. B., Seltzer, C. C., Feldman, R. & Collen, M. F., 1973. Arch Environ Health 26: 137–143.
Rodriguez, R. J., White, R. R., Senior, R. M. & Levine, E. A., 1977 Science 198: 313–314.
Hoidal, J. R., Fox, R. B., LeMarbe, P. A., Perri, R. & Repine, J. E., 1981. Am. Rev. Respir. Dis. 123: 85–89.
Corberand, J., Nguyen, F., Do, A. H., Dutau, G., Laharrague, P., Fontanilles, A. M. & Gleizes, B., 1979. Infect. Immun. 23(3): 577–581.
Corberand, J., Laharrague, P., Nguyen, F., Dutau, G., Fontanilles, M., Gleizes, B. & Gyrard, E., 1980. Infect. Immun. 30(3): 649–655.
Neidel, J., Wilkinson, S. & Cautrecasas, P., 1979. J. Biol. Chem 254: 10700–10706.
Richman, D. P. & Arnason, B. G. W., 1979. Proc. Natl. Acad. Sci. 76(9): 4632–4635.
Schwartz, R. H., Bianco, A. R., Handwerger, B. S. & Kahn, C. R., 1975. Proc. Natl. Acad. Sci. 72(2): 474–478.
Lopker, A., Abood, L. G., Hoss, W. & Lionetti, F. J., 1980. Biochem. Pharmacol. 29: 1361–1365.
Williams, L. T., Snyderman, R. & Lefkowitz, R. J., 1976. J. Clin. Invest. 57: 149–155.
Lionetti, F. J., Hunt, S. M., Lin, P. S., Kurtz, S. R. & Valeri, C. R., 1977. Transfusion 17: 465–472.
Hunt, S. M., Lionetti, F. J., Valeri, C. R. & Callahan, A. B., 1981. Blood 57: 592–598.
Aguado, T., Prijol, N., Rubiol, B., Trura, M. & Celads, A., 1980. J. Immunol. Methods 32: 41–50.
Dankberg, F. L., Persidsky, M. D., 1976. Cyrobiology 13: 430–440.
Barbeau, A., Campanella, G., Butterworth, R. F. & Yamada, K., 1975. Neurology 25: 1–9.
Galant, S. P., Duriseti, L., Underwood, S. & Insel, P. A., 1978. N Engl. J. Med. 299: 933–936.
Pert, C. B. & Synder, S. H., 1975. Mol. Pharmacol. 11: 735–744.
Aronstam, R. S., Abood, L. G. & Hoss, W., 1978. Mol. Pharmacol. 14: 575–586.
Ignarro, L. J. & George, W. J., 1974. J. Exp. Med. 140: 225–231.
Russell, M. A. H., Jarvis, M., Iyer, R. & Feyerabend, C., 1980. Br. Med. J.: 972–976.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Davies, B.D., Hoss, W., Lin, JP. et al. Evidence for a noncholinergic nicotine receptor on human phagocytic leukocytes. Mol Cell Biochem 44, 23–31 (1982). https://doi.org/10.1007/BF00573842
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00573842