Article Text
Abstract
Background and aims: Activation of the vanilloid receptor subtype 1 (VR-1) results in release of proinflammatory peptides which initiate an inflammatory cascade known as neurogenic inflammation. We investigated its role in an acute model of surgically induced oesophagitis.
Methods: Oesophagitis was induced by pyloric ligation in wild-type and VR-1 deficient mice. A subset of animals were administered the VR-1 antagonist capsazepine, famotidine, or omeprazole one hour before surgery. Five hours after surgery, myeloperoxidase activity (MPO), histological damage scores, intragastric pH, and immunocytochemical analysis of substance P (SP) receptor endocytosis were determined.
Results: Oesophagitis induced knockout mice exhibited significantly lower levels of MPO activity, histological damage scores, and SP receptor endocytosis than wild-type mice. Inflammatory parameters were significantly reduced by acid inhibition and capsazepine in wild-type mice.
Conclusions: We conclude that acute acid induced oesophagitis is reduced in animals lacking VR-1. This suggests that acid induced oesophagitis may act through VR-1 and that inhibition of the receptor may reduce inflammation.
- VR-1, vanilloid receptor subtype 1
- GORD, gastro-oesophageal reflux disease
- MPO, myeloperoxidase
- NK-1, neurokinin 1
- SP, substance P
- PCR, polymerase chain reaction
- HTAB, hexadecyltrimethyllammonium bromide
- receptor
- substance P
- neurogenic inflammation
- oesophagitis
- vanilloid receptor subtype-1
- capsaicin
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Footnotes
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Conflict of interest: None declared.
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